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Description

To explore the underlying pathophysiological mechanisms of increased production of surface reactive oxygen species in AD, HD, and amyotrophic lateral sclerosis (102)

Lysates were incubated on ice for 20 minutes, cleared from insoluble debris by centrifugation (10,000 x g , 30 minutes, 4 C), loaded onto glutathione Sepharose 4B beads (GE Healthcare, #17-0756-01) and incubated for 24 h at 4 C