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10.1074/jbc.M109.003301 J

Electrophoretic techniques were used to examine native protein and isoenzyme patterns

The final reaction comprises of 2 ml of Na-phosphate buffer (pH 7.3, 0.5 M,) 0.1 ml of enzyme extract, 0.1 ml of EDTA (3 mM) and 0.1 ml of H 2 O 2 (3 mM) for 5 min