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The next day, cell mixtures were scraped and resuspended in 1 ml LB and 1000X dilutions of this conjugation mixture were plated on 10 large (150 mm 15 mm) LB plates (lacking DAP) supplemented with 50 g/ml kanamycin

In Huh7 cells, E2 expression was also a modulator of IRS-1 by impairing its insulin-induced phosphorylation and the phosphorylation of GSK3, leading to an inhibition of glucose uptake and glycogen synthesis, respectively (87)

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Although GPX4 depletion alone failed to affect HSCs function in vivo , Vav1- Gpx4 knockout mice fed with a vitamin E-depleted diet exhibited a reduced number of HSCs and dysregulated hematopoiesis, mainly due to increased ferroptosis [22]