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Ten ul of CCK-8 solution in 100 ul culture medium was added to the wells before a 2 h incubation at 37 C

Effects of human GH and its lipolytic fragment (AOD9604) on lipid metabolism in obese mice and beta(3)-AR knock-out mice Endocrinology, 2001, foundational beta-3 adrenergic and lipolysis study PMID 15134286 Wilding J

Patients with conditions showing strong preclinical evidence and limited alternative treatment options may represent reasonable candidates following thorough informed consent discussions