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4 Chemical pretreatment techniques in tobacco stalk Unlike other conventional lignocellulosic biomass, the pretreatment of tobacco stalk lignocellulose must address not only the barriers caused by the tightly bound structures of lignocellulosic and cell wall components (lignin, hemicellulose, and cellulose) to facilitate efficient enzymatic production of fermentable sugars but also the removal of toxic substances such as nicotine from the biomass (Sun et al., 2020)

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