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3 uL of sample was applied to grids, blotted for 3 s with a blot force of 15 at 100% humidity and 22 C, and frozen in ethane using a Vitrobot Mark IV (Field Electron and Ion Company)

The consequences seem to be highly dependent on the degree of L-glu increase but even a 10% increase appears to affect nerve cell structure and survival particularly in the context of aging suggesting that chronic excitotoxicity may be particularly relevant to age-related neurodegenerative diseases