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GSH depletion can trigger ferroptosis, which has been exploited in cancer treatments [17], and supplementation with GSH is utilized by glutathione peroxidase 4 (GPX4) to prevent lipid peroxidation, in turn inhibiting ferroptosis

Phase Split option Daily dose 50 mg morning + 50 mg midday Notes Some sources prefer split AM dosing because the plasma half-life is short (about 4 to 7 hours in rodent PK)

We review substrate handling, activation strategy, order of addition, and buffer or solvent compatibility so the reaction is optimized for the chemistry actually present rather than for a generic GSH workflow

Break periods between cycles Between AOD-9604 cycles, a break period allows receptor sensitivity to restore and prevents potential adaptation that could reduce effectiveness in future cycles