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Biomarkers in colorectal liver metastases

J Am Acad Orthop Surg

Analyzed were karyopyknotic cells in the affected brain areas (04), cerebral (NTP-7, Level 3), cerebellar cortex (NTP-7, Level 6), and hippocampus, thalamus, and hypothalamus (NTP-7, Level 3) as follows (score 0 indicates no change): score 1: a few karyopyknotic of neuronal cells (20%)

After hydrolysis, 20 L of each sample was neutralized by the addition of 20 L of 6 M NaOH and 20 L of 0.2 mM HCl, and then filtered using a 0.45 m nylon syringe filter