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Effects of AKG and GDF9 on cumulus cells expansion Sequencing results showed that the expression of cumulus expansion-related genes, including HAS2 , PTX3 , CD44 and HMMR , were not significantly altered in the 30 M AKG treatment group but was significantly downregulated in cumulus cells treated with 750 M AKG compared to the control group (Fig

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Notably, most of the affinity to the binding site was still maintained

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