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d , Cell viability of HT1080 GPX4 KO/FSP1OE cells transduced with either a non-targeting control (NT) or three different RFK -targeting sgRNAs in the absence or presence of Lip-1 (500 nM)

This work was supported by NIH grant HD 048867 to PS

Glutathione agarose was first washed with 500 L of buffer W at 4 C and then with 500 L of buffer WT (100 mM TrisHCl [pH 7.5], 150 mM NaCl, 1 mM EDTA.2Na [pH 8.0], and 10% of Triton X-100)