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36 showed in an animal model that a methionine and choline-deficient diet resulted in significant changes in serum glutamine levels, and other studies reported a switch from the GLS2 to the GLS isoform in the end stages of chronic liver disease

Next to promoting microthrombus formation, platelets play a critical role in the development of DCI by amplifying the neuro-inflammatory response through the secretion of proinflammatory mediators leading to leukocyte recruitment and triggering cortical spreading depolarizations through the release of glutamate (34)

The RT-PCR reinforcement was carried out by AddScript RT-PCR SYBR Master (2x conc.) (Addbio, Korea)

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