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Acrolein was also shown to activate the nuclear factor erythroid 2-related factor 2 (Nrf2) transcription factor [75]

Weekly serum samples and BAL fluid from individual mice were added to blocked plates and bound anti-P6 Ig were detected with HRP-conjugated goat anti-mouse Ig(H+L), IgA, IgG1, IgG2a, IgG2b (Southern Biotech)

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