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Tissue preparation and immuno-staining retinal flat-mounts with TH and quantification of cell bodies One eye from each animal was fixed in 4% PFA for 4 h followed by one wash in PBS and eyes prepared for flat mount preparation as described for lectin staining

768-779) Kwon I, Xiang S, Kato M, Wu L, Theodoropoulos P, Wang T, Kim J, Yun J, Xie Y, McKnight SL, Poly-dipeptides encoded by the C9ORF72 repeats bind nucleoli, impede RNA biogenesis, and kill cells, Science 2014, Published online 31 July 2014 [DOI:10.1126/science.1254917] Chu Y, Wang T, Dodd D, Xie Y, Janowski BA, Corey DR

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