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The PBMCs were stained with GhostDye Violet 450 viability dye (Tonbo Biosciences, San Diego, CA) for 30 min at 4C, washed once with PBS containing 2% FBS and 0.05% sodium azide (PFN), and blocked with anti-human CD32 Clone IV.3 (StemCell Technologies, Vancouver, BC) for 15 min at 23 C

A top-fill design has advantages over a side-fill like the older U-Series pod

This can lead to substantial problems across a range of issues in treatment courses for individuals with SUDs, including neurocognitive function after methamphetamine use, overlapping or additional SUDs, and patient social networks and finances