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Besides ddPCR-based techniques, the advent of novel high-throughput technologies such as sequencing approaches (e.g., next-generation sequencing and pyrosequencing) and microarray (e.g., Infinium HumanMethylation450k Bead Chip array and Infinium Human MethylationEPIC array by Illumina) has revolutionized the analysis of DNA methylation at single-nucleotide resolution [89, 90]

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