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The medium was replaced, and after 8 h the cell supernatant was collected and co-incubated with RAW264.7 cells for 4 h

The anti-calcific effect of zinc on human valve interstitial cells (hVIC) calcification is, at least in part, mediated through the inhibition of apoptosis and osteogenic differentiation via the GPR39-dependent ERK1/2 signaling pathway