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Generating human ACE2 over-expressing cells Wildtype ACE2 sequence was obtained from Ensembl Gene Browser (Transcript ID: ENST00000252519.8) and codon-optimized with SnapGene by removing restriction enzyme recognition sites that are necessary for subsequent molecular cloning steps, preserving the amino acid sequence

The pattern that should be used and modeled after is the PREEMPT protocol ( P hase III RE search E valuating M igraine P rophylaxis T herapy), based off the trial that led to FDA approval for Botox in the prevention of chronic migraine

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