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It should be noted that these studies measured total Cbl levels, but as previously mentioned, the biologically available form of Cbl, TCN2-Cbl, comprises only approximately 2030% of the total Cbl level, which may lead to a significant number of false negatives for clb deficiency

Transdermal patches and nasal sprays Both transdermal B12 patches (patches that stick to the skin) and B12 nasal sprays are relatively new to the market and are not commonly prescribed by doctors

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For quantification of [ 15 N 13 ]-CNCbl, the influence of trace CNCbl originating from the endogenous cobalamins in the soybean flour was negligible due to addition of high amounts of exogenous CNCbl as internal standard