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doi: 10.1016/j.cophys.2022.100483

During the early postnatal period (P1-P20) (Figure 2), the vitamin C recycling mechanism is established between recently differentiated astrocytes and maturating neurons

Lysates from C2C12 cells transiently transfected to express GFP-Dp71f and their different variants carrying NES1/NES2 mutations, were subjected to GFP-based immunoprecipitation assays (see Methods), and the precipitated proteins were analyzed by Western blot using antibodies against CRM1 and GFP (loading control)