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In addition, TRPM2 contains a structure of ADP-ribose (ADPR) pyrophosphatase enzyme, although this domain of hTRPM2 is catalytically inactive (Iordanov et al., 2016), and is activated by cytosolic ADPR (cADPR) and reactive oxygen species (ROS) (Hara et al., 2002)

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However, pre-culture seemed to reduce metabolic competence, as CYP2E1 activity was not detected using a CYP2E1-specific substrate, although mRNA expression was confirmed using qRT-PCR