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The analysis of cysteine, glutathione and phytochelatins is performed at 0, 3, 7, 14 and 21 days

With a little thoughtful planning, affinity tags can make protein purification a cinch

Abbreviations NAFLD: Non-alcoholic fatty liver disease HFD: High-fat diet PPAR: Peroxisome proliferator-activated receptor gamma ATGL: Adipose triglyceride lipase ACAD: Acyl CoA dehydrogenase FAS: Fatty acid synthase CPT1: Carnitine palmitoyltransferase 1 NASH: Nonalcoholic steatohepatitis FMN: Flavin mononucleotide FAD: Flavin adenine dinucleotide GR: Glutathione reductase PA: Palmitic acid DMSO: Dimethyl sulfoxide BSA: Bovine serum albumin ALT: Alanine aminotransferase AST: Aspartate aminotransferase TG: Triglycerides TC: Total cholesterol SOD: Superoxide dismutase GSH-Px: Glutathione peroxidase C: Control HFRD: High-fat riboflavin-deficient diet ELISA: Enzyme immunosorbent assay NAS: NAFLD activity score RT-PCR: Reverse transcription-polymerase chain reaction siRNA: Small interfering RNA References Tarantino G, Citro V, Capone D

Vitamin C (Ascorbic Acid and its derivatives): Fights free radicals and enhances skin radiance