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Bastiaansen et al., in vivo with high sensitivity and a time resolution of 1 s, the acquisition window is limited to approximately a minute (Comment, 13 C in vivo to quantification of metabolic fluxes (Bastiaansen et al., The strong heteronuclear scalar coupling between 13 C and 1 H nuclei complicates the spectra (i.e., splitting of 13 C resonances in multiplets with reduced peak height) and reduces the sensitivity, so that additional hardware is required for 1 H decoupling during acquisition (discussed in Henry et al., 13 C couplings are also observable and the presence of 13 C multiplets depends on the simultaneous presence of 13 C spins in the same molecule at adjacent positions

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Bioresource Technol 107:1924

Tavakoli S, Klar AS