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Elevated TMAO levels have been linked to a number of diseases, including cardiovascular and neurological disorders, renal disease, metabolic syndrome, obesity, type 2 diabetes (T2D), non-alcoholic fatty liver disease (NAFLD), cancer, and severe COVID-19

found after acupuncture treatment in PD mice, a reduction in Proteobacteria in the gut, indicating decreased TMAO levels (Jang et al., 2020) (Figure 8)

In these mice, a significant reduction in the number of c-Fos-positive orexin neurons, hypothalamic prepro-orexin mRNA expression, and orexin-A concentration in the cerebrospinal fluid (CSF) was observed [19], [21]

(R)- and (S)-ketamine induce differential fMRI responses in conscious rats