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Possible side effects include[10]: Bloating or abdominal discomfort Nausea or vomiting Diarrhea Headaches Dizziness or feeling lightheaded Flushing or low blood pressure Rare but serious reactions, such as allergic responses or irregular heartbeat, have been reported, especially in individuals with heart conditions

All the other chemicals and reagents were bought from reputed manufacturers of analytical grade

TME is a complex ecosystem composed of tumor cells, extracellular matrix (ECM), innate and adaptive immune cells, inflammatory cells, tumor-associated macrophages (TAMs), cancer-associated fibroblasts (CAFs), tumor-associated neutrophils, myeloid-derived suppressor cells (MDSCs), endothelial cells, microvascular networks, as well as various cytokines and chemokines (Wu et al., 2022)