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2021 Feb 27;22(5):2394

It works as cellular fuel, converting food into energy through a process called mitochondrial respiration

Bead pools were resuspended in BSS (500 mM Tris-HCl pH 7.5, 1.5 M NaCl, 50 g/L BSA) and incubated in absence or presence of 10 nM TCEs at 37 C for varying amounts of time, as reported in the figures (typically 24, 8, 4, 2, 1, 0.5, 0.25 h)

AB staining identified sperm cells with abnormal chromatin packaging (dark blue nuclei), whereas AO staining distinguished between spermatozoa with normal DNA (green) and damaged DNA (orange/red) 99