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Reactions were incubated at 30 C for 1 h and then terminated by the addition of sodium dodecyl sulfate (SDS) sample buffer

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Conversely, in IL-6 trans-signaling, soluble forms of IL-6R (sIL-6R), produced through either alternative splicing or limited proteolytic processing, form complexes with IL-6, thereby initiating the activation of membrane-bound gp130

Braz J Pharm Sci 58:e19517 Mackonochie M, Rodriguez-Mateos A, Mills S, Rolfe V (2023) A scoping review of the clinical evidence for the health benefits of culinary doses of herbs and spices for the prevention and treatment of metabolic syndrome