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For ligand preparation, hPD-L1 protein was diluted in 10 mM sodium acetate buffer (pH 4.5) to achieve a working concentration of 5 g/mL, resulting in a final immobilization level of 800 response units (RU) after surface activation

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Inject slowly over 1015 seconds

By following best practices for sterility, storage, and administration, researchers can maximize the effectiveness of their studies and contribute valuable insights into the role of human growth hormone fragments in fat metabolism and energy regulation