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Aging negatively skews macrophage Tlr2- and Tlr4-mediated pro-inflammatory responses without affecting the il-2-Stimulated pathway

Also, as a negative control for the assay, PbWT yeast cells previously treated with 16 g/ml of amphotericin B during 4h (Fungizone, Bristol-Myers Squibb Pharmaceuticals, England) were used [57]