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First, they must be transported to the mitochondria where the enzymes of -oxidation are located and fatty acids are oxidized, as there is only a minimal contribution from the peroxisomal system for which the preferred substrates are very long-chain fatty acids

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In brief, peptides from STC-1 cells were generated using a Single-Pot Solid-Phase-enhanced Sample Preparation (SP3) protocol, 187 with minor modifications

The prepared sample (6 ml) was then loaded onto equilibrated SPE cartridges and allowed to flow through under gravitation