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The homogenate was filtered through a 100-m cell strainer and centrifuged at 500 g , followed by transfer of the floated adipocytes to an Eppendorf tube for RNA isolation

J Sex Med 14(12):15751584 Cully M, You H, Levine AJ, Mak TW (2006) Beyond PTEN mutations: the PI3K pathway as an integrator of multiple inputs during tumorigenesis