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Real time quantitative PCR Total RNA was obtained from 1.5 mL of aliquots from endpoints Z, A and B, using the QIAamp RNA Blood kit (Qiagen, Hilden, Germany), and cDNA synthesis was performed from 2 g of total RNA using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher, Waltham, Massachusetts), both according to manufacturers protocols

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Thrombocytopenia may be identified during blood work ordered by a primary care provider, including internists, pediatricians, and family medicine specialists

Vitamin B6 is necessary for the production of serotonin and dopamine, which are neurotransmitters dedicated to regulating our moods and stabilising our emotions