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Documentation and tracking Keep a simple log for each vial: reconstitution date, water volume added, concentration, and a running tally of doses drawn with dates

2C illustrates mammalian expression of SpCas9 and SpRNase III driven by the constitutive EF1a promoter and tracrRNA and pre-crRNA array (DR-Spacer-DR) driven by the RNA Pol3 promoter U6 to promote precise transcription initiation and termination