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Blood sugar regulation for cardiovascular health promotion and disease prevention: JACC Health Promotion Series

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After incubation, mitochondria were pelleted (1 min at 16,000 g ) and pellets were resuspended in 200 L of 50 mM HEPES buffer (pH 7.4) supplemented with 10 mM IAM-TPP, disrupted using mechanical micro-homogeniser and sonicated for 5 min

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