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Furthermore, reduced SNCA protein level by CRISPR-dCas9-DNMT3A system rescued disease-related cellular phenotypic features of SNCA triplicated dopaminergic neurons, such as mitochondrial ROS production and low cell viability (Wllner et al., 2016)

Acquired immunity was found to be mainly associated with a Th1 type IFN- and TNF pulmonary inflammatory and antibody response, IL-4, IL-5, and eosinophils are of negligible importance meanwhile, IL-10 and IgG4 negates protection and are associated with re-infection ( Utilizing advancements in technology, vaccine development has, in the recent past, shifted focus from irradiated parasite vaccines to specific antigen molecules

Viral supernatants were collected 48 and 72 h post-transfection, filtered through 0.45 m filters (Millipore, SLHV033RS)