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Figure 4a briefly illustrated the experimental scheme

Transcriptome profiling of sulfur-responsive genes in Arabidopsis reveals global effects of sulfur nutrition on multiple metabolic pathways

A healthy, unstressed cell maintains a vast majority of its glutathione pool in the reduced state, typically sustaining a GSH-to-GSSG ratio of greater than 10:1, and often up to 100:1 in the cytosol

During the ER stress regulation, ATF4 activates UPR target gene, which encodes proteins necessary for antioxidant reaction and amino acid synthesis and transport [120]