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Cytosolic -oxidation generates short-chain fatty acids that contain -hydroxyl groups, 1-hydroxyl groups, or ,-unsaturated fatty acids that have been identified in the literature as genotoxic substances (2-propyl-4-pentenoic acid and 2-propyl-3-pentenoic acid) that could also promote hyperammonemia

The authors, however, did not carry out detailed histopathological studies to support their results

Sorted single-cell suspensions were resuspended at an estimated final concentration of 1000 cells/l and loaded on a Chromium GemCode Single Cell Instrument (10x Genomics) to generate single-cell gel beads-in-emulsion (GEM)