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RB: Methodology, Investigation

Following that, the tissue samples were immersed in graded concentrations of sucrose buffer (1030%) for cryoprotection at 4 C overnight

Acknowledgements: CRAM:Research Ireland:13/RC/2073_P2,SENSOME Disclosure of interest: All authors:nothing to disclose 1 - ICH & SAH 02.00 - INTRACRANIAL HEMORRHAGE - 02.01 - INTRACEREBRAL HAEMORRHAGE P740 - ESOC25-498 HEMATOMA LOCATION AND SYMPTOM ONSET TO SAMPLING ARE PREDICTORS OF GFAP RELEASE INTO PLASMA IN ACUTE INTRACEREBRAL HEMORRHAGE Sabina Zylyftari 1 , Love-Preet Kalra 1 , Sebastian Luger 1 , Stephan Barthelmes 1 , Kristaps Blums 1 , Christian Foerch 1 1 RKH Klinikum Ludwigsburg, Ludwigsburg, Germany Background and Aims: GFAP (glial fibrillary acidic protein) is a promising biomarker for distinguishing intracerebral hemorrhage (ICH) from ischemic stroke in the prehospital phase