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It is, therefore, imperative to safeguard the function of mitochondria and prevent any potential dysfunction to effectively treat diseases such as DED (Lpez-Lluch, 2021)

Subsequent examination after eight days of co-culture revealed that intestinal organoids derived from the co-culture of ISCs and old Paneth cells exhibited aging characteristics compared to those from the co-culture of ISCs and young Paneth cells, evidenced by a notable reduction in intestinal organoids and organoid bud formations (Fig

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