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35 g of proteins were loaded on the separating 10 or 12% acrylamide/bis-acrylamide SDS-PAGE and transferred onto a nitrocellulose membrane (Millipore, Billerica, MA, USA), which was then blocked for 30 min in a 5% non-fat milk powder in a PBS solution and incubated overnight at 4 C with the primary antibody

Mar Drugs 9(3):447465 Montezano AC, Touyz RM (2012) Reactive oxygen species and endothelial functionrole of nitric oxide synthase uncoupling and No x family nicotinamide adenine dinucleotide phosphate oxidases

1 It may be difficult, however, to measure exactly how much L-arginine you are consuming compared to a commercially available supplement