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Description

, Subramanian P., and Jayakumar M

Nonalcoholic fatty liver disease is associated with an increased prevalence of distal symmetric polyneuropathy in adult patients with type 1 diabetes

fuscopurpurea Actin gene as an internal reference (Livak and Schmittgen, 2001)

Amplicons from the 2 nd round were gel-purified by electrophoresis (3% agarose gel, 4.2 V/cm, 2 hours) and quantified using the 1X dsDNA HS kit with the Qubit 4.0 Fluorometer (Invitrogen)