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Isothermal titration calorimetry (ITC) Recombinant and synthetic proteins were used in ITC buffer (20 mM HEPES, pH 7.4, 150 mM NaCl, 0.5 mM TCEP-HCl)

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Briefly put, raw sequencing reads were stored as FASTQ files and then aligned to the human reference genome (UCSC hg19) with the Burrows-Wheeler alignment [25]