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This finding implies that Dubosiella may be essential for controlling metabolic homeostasis, which is dependent on ovarian hormones ( Dubosiella plays a role in the occurrence and development of PCOS through multiple pathways, such as affecting intestinal barrier function, short-chain fatty acid production, inflammatory pathways, and bile acid metabolism (Qian et al., 2026)

( L ) Western blotting (top) and quantitative analysis (bottom) of GPX4 and TFRC expression in midbrain, cortex, and hippocampus, respectively

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Biotinylated (bt)-miR-mRNA pulldown (PD) experiments The PD assay was performed to enrich for high-specificity miRNA targets (Tan & Lieberman, 2016