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These results are consistent with the major features of hind-limb suspension-related phenotypes of skeletal muscles as an index of anti-gravity muscle atrophy 66 , thus assuring that the experimental design including the mouse age and muscle-disuse period (in vivo assay time-point) were appropriate to the conclusion that 3-days LASSS oral-administration prevents tyrosine-residue nitration of extracellular HGF (Y198) during 5-days muscle disuse

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For purification of endogenous NINJ1 or NINJ1-V5, HT-1080 cells were lysed in NP-40 buffer with protease inhibitors, followed by constant vortexing at 4 C and centrifugation