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T2DM and T2DM-CKD patients exhibit increased gut microbiota TMA-producing bacteria and significantly higher serum TMAO, positively correlating with zonulin, LPS, inflammation, and endothelial dysfunction biomarkers (148)

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Lastly, the International Restless Leg Syndrome Study Group rating scale (IRLSS) appraises the presence and severity of RLS and is a valuable tool specifically in patients with primary biliary cholangitis (31, 33, 36)

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